Product Description

Simian OCLN (Occludin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11642-48, ELK11642-96, ELK11642-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: Tight Junction Protein Occludin TM4 Minus

Sensitivity: 0.054 ng/mL

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OCLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OCLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OCLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only