Product Description

Simian TRACP-5b (TartSimiane Resistant Acid Phosphatase 5b) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11611-48, ELK11611-96, ELK11611-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: ACP5,TRAP5b,SPENCDI

Sensitivity: 0.45 ng/mL

Assay Type: Sandwich

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Signal Transduction, Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only