Product Description

SIRT3 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5340-50, ES5340-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: SIRT3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human SIRT3. AA range:350-399

Isotype: IgG

Target: SIRT3

Specificity: SIRT3 Polyclonal Antibody detects endogenous levels of SIRT3 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed,

Observed Band (KD): 45kD

Fields: Nicotinate and nicotinamide metabolism, Metabolic pathways, Central carbon metabolism in cancer

Function: Catalytic Activity: NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein
Cofactor: Binds 1 zinc ion per subunit
Function: NAD-dependent deacetylase. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo
PTM: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity
Similarity: Belongs to the sirtuin family
Similarity: Contains 1 deacetylase sirtuin-type domain
Tissue Specificity: Widely expressed

Subcellular Location: Mitochondrion matrix

Storage and Stability: -20°C/1 year

Gene Name: SIRT3

Protein Name: NAD-dependent protein deacetylase sirtuin-3 mitochondrial

Mouse SwissProt Number: Q8R104

Research Use Only