Product Description

SMCA5 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES13053-50, ES13053-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human SMCA5 AA range: 851-901

Isotype: IgG

Target: SMCA5

Specificity: This antibody detects endogenous levels of SMCA5 at Human/Mouse

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed,

Molecular Weight (Da): 116kD

Function: Developmental Stage: Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy
Function: Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin, this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the

Subcellular Location: Nucleus Chromosome Localizes to mitotic chromosomes (PubMed:12972596) Co-localizes with RSF1 in the nucleus (PubMed:12972596) Co-localizes with PCNA at replication foci during S phase (PubMed:15543136) Co-localizes with BAZ1B/WSTF at replication foci during late-S phase (PubMed:15543136) Recruited to DNA damage sites following interactiuon with SIRT6 (PubMed:23911928)

Storage and Stability: -20°C/1 year

Gene Name: SMARCA5 SNF2H WCRF135

Protein Name: SMCA5

Mouse Gene ID: 93762

Mouse SwissProt Number: Q91ZW3

Research Use Only