SPH (Sphingomyelin) ELISA Kit - ELK8171

SPH (Sphingomyelin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8171-48, ELK8171-96, ELK8171-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SM

Assay Type: Competitive Inhibition

Sensitivity: 1.82 µg/mL

Standard: 400 µg/mL

Detection Range: 6.25-400 µg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Neuroscience;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only