Product Description

SPOP Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8522-50, ES8522-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Speckle-type POZ protein (HIB homolog 1) (Roadkill homolog 1)

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human SPOP. AA range:41-90

Isotype: IgG

Target: SPOP

Specificity: SPOP Polyclonal Antibody detects endogenous levels of SPOP

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed,

Observed Band (KD): 42kD

Fields: Hedgehog signaling pathway

Function: Domain: The MATH domain interacts with H2AFY and BMI1
Function: Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3
Miscellaneous: Antigen recognized by serum from scleroderma patient
Pathway: Protein modification, protein ubiquitination
Similarity: Belongs to the Tdpoz family
Similarity: Contains 1 BTB (POZ) domain
Similarity: Contains 1 MATH domain
Subunit: Homodimer. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1, BMI1 and DAXX. Interacts with CUL3
Tissue Specificity: Widely expressed

Subcellular Location: Nucleus Nucleus speckle

Storage and Stability: -20°C/1 year

Gene Name: SPOP

Protein Name: SPOP

Mouse Gene ID: 20747

Mouse SwissProt Number: Q6ZWS8

Research Use Only