Streptavidin Sepharose High Performance affinity resin
Size: 5 mL
Catalog Number: St-383C
Citations, Manuals and MSDS Available upon request.
Product Type: Chromatography Reagents
Storage Temperature: Store unused media in its container at a temperature of 2 to 8°C for long term storage.
Applications: The interaction between streptavidin and biotin is very strong and requires denaturing conditions for elution. This strong interaction can be utilized in the purification of antigens. Biotinylated antibody-antigen complexes bind to the HiTrap Streptavidin HP column, enabling subsequent elution of the antigen. An alternative application is to exploit the interaction between 2-iminobiotin and streptavidin, which is a weaker interaction. Iminobiotinylated substances can be eluted from the column at pH 4.
Research Use Only