Product Description

Succinyl-CoA Synthetase (SCS) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K906-M-48, E-BC-K906-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Succinyl-CoA synthetase (SCS) is a key enzyme in the citric acid cycle and an important metabolic intermediate in the biosynthesis of porphyrins, hemes, and ketones. SCS converts succinyl-coenzyme A (CoA-SH) to succinic acid. The reaction between CoA-SH and pyruvate produces chromogenic substances with a characteristic absorption peak at 450 nm. The activity of SCS can be calculated by measuring the change of absorbance value at 450 nm.

Abbreviation: SCS

Research Areas: Glycolysis and Lipid Metabolism;Tricarboxylic Acid（TCA）Cycle;Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.09-20.72 U/L

Tested Sample Types: Tissue;Cell

Assay Length: 1 h

Sensitivity: 0.09 U/L

Inter cv: 4.9-10.3%

Intra cv: 2.5-8.0%

Research Use Only