Product Description

TCPa (Alpha-Tocopherol) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7865-48, ELK7865-96, ELK7865-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TCPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TCPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TCPa in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: VE, Vitamin E, α-TCP, α-Tocopherol

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Nutrition Metabolism

Assay Length: 2h

Sensitivity: 0.45 µg/mL

Standard: 100 µg/mL

Detection Range: 1.57-100 µg/mL

Research Use Only