THB (Tetrahydrobiopterin) ELISA Kit - ELK9249

THB (Tetrahydrobiopterin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9249-48, ELK9249-96, ELK9249-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Tetrahydrobiopterin; BH4; Kuvan; Sapropterin

Assay Type: Competitive Inhibition

Sensitivity: 0.53 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Genetic diseases

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with THB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THB in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only