Product Description

THFA (Tetrahydrofolic Acid) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8034-48, ELK8034-96, ELK8034-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with THFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THFA in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: H4FA, THF, Tetrahydrofolate

Reactivity: General

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Enzyme and Kinase

Assay Length: 2h

Sensitivity: 0.56 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Research Use Only