Product Description

Tissue Total Cholesterol (TC) Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K1103-M-48, E-BC-K1103-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Total cholesterol (TC) includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase (CE) produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

Abbreviation: TC

Research Areas: Metabolic Diseases,Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.29-24 mmol/L

Tested Sample Types: Animal tissue

Assay Length: 30 min

Sensitivity: 0.29 mmol/L

Inter cv: 4.5%-8.3%

Intra cv: 2.0%-3.1%

Research Use Only