Product Description

Total Bile Acid (TBA) Colorimetric Assay Kit

Sizes: 48T, 96T, 500Assays

Catalogue Numbers: E-BC-K181-M-48, E-BC-K181-M-96, E-BC-K181-M-500

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: With S-NAD+as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

Abbreviation: TBA

Target Synonym: TBA

Research Areas: Liver and Renal Function

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 1.77-40 μmol/L

Tested Sample Types: Serum;Animal tissue

Assay Length: 25 min

Sensitivity: 1.36 μmol/L

Inter cv: 3.7%

Intra cv: 3.4%

Research Use Only