Product Description

Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

Sizes: 48T, 96T, 500Assays

Catalogue Numbers: E-BC-K109-M-48, E-BC-K109-M-96, E-BC-K109-M-500

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce hydrogen peroxide. In the presence of Trinder's reagent, hydrogen peroxide is catalyzed by peroxidase (POD) to produce a red quinoneimine dye, whose color intensity is directly proportional to the TC concentration and can be measured at 510 nm.

Abbreviation: TC

Target Synonym: TC

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.29-25.85 mmol/L

Tested Sample Types: Serum;Plasma;Tissue

Assay Length: 20 min

Sensitivity: 0.29 mmol/L

Inter cv: 8.3%

Intra cv: 3.1%

Research Use Only