Product Description

Total Phosphodiesterase (PDEs) Acitivity Colorimetric Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-K782-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Phosphodiesterase (PDEs) hydrolyzes the intracellular second messenger (cyclic adenosine phosphate or cyclic guanosine phosphate), thereby ending the biochemical effects of these second messengers.
The principle of this kit is that by hydrolyzing the substrate with total PDEs, the resulting substance has a maximum absorption peak at 405 nm. The OD value at 405 nm and the standard curve were measured to calculate the total PDEs activity in the sample.

Abbreviation: PDEs

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.16-16.64 U/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 1 h 20 min

Sensitivity: 0.16 U/L

Inter cv: 4.1-5.6%

Intra cv: 1.2-3.0%

Research Use Only