Product Description

Troponin I-C (phospho Ser22/S23) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4389-50, ES4389-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Reactivity: Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin I

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54

Isotype: IgG

Target: Troponin I-C

Specificity: Phospho-Troponin I-C (S22/S23) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at S22/S23.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Observed Band (KD): 28kD

Storage and Stability: -20°C/1 year

Gene Name: Tnni3

Protein Name: Troponin I cardiac muscle

Mouse Gene ID: 21954

Rat Gene ID: 29248

Rat SwissProt Number: P23693

Research Use Only