Tubulin α (G436) Monoclonal Antibody-BS1699M

Tubulin α (G436) Monoclonal Antibody

Catalogue Numbers: BS1699M-50, BS1699M-100

Sizes: 50µl, 100µl

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q71U36/P68363

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:5000~20000
IHC: 1:100~500
IF: 1:100~500

Background: Tubulin is a major cytoskeleton component that has three distinct forms, designated α, β and γ Tubulin. α and β Tubulins form heterodimers, which multimerize to form a microtubule filament. γ Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The α and β isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II β Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Tubulin α (G463) mAb detects endogenous levels of α-Tubulin protein.

Extra Notes: Western blot (WB) analysis of Tubulin α（G436）pAb at 1:5000/1:10000 dilution
Lane1:HEK293T whole cell lysate(20ug)
Lane2:C6 whole cell lysate(20ug)
Lane3:BV2 whole cell lysate(20ug)
Lane4:The Heart tissue lysate of Mouse(20ug)
Lane5:The Brain tissue lysate of Rat(20ug)

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1A; TUBA3; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B

Immunogen: Synthetic peptide, corresponding to amino acids 201-256 of Human Tubulin α.

Conjugate: Unconjugated

Modification: Unmodified