Tubulin β (4D9) Monoclonal Antibody-HRP-BS1482MH

Tubulin β (4D9) Monoclonal Antibody-HRP

Sizes: 50µl, 100µl

Catalogue Numbers: BS1482MH-50, BS1482MH-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13509/P04350/P68371/P07437/Q13885/Q9BUF5

Host: Mouse

Reactivity: Human, Mouse, Rabbit, Frog, Fish, Rat (not applicable to some rat tissue)

Applications: WB, IHC

All Applications: WB: 1:10000
IHC: 1:100~1:1000

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Tubulin β (4D9) monoclonal antibody-HRP at 1:10000 dilution
Lane 1: The Brain tissue lysate of Rat (10ug)
Lane 2: The Brain tissue lysate of Mouse (10ug)
Lane 3: PC12 whole cell lysate (10ug)
Lane 4: BV2 whole cell lysate (10ug)
Lane 5: A549 whole cell lysate (10ug)

Alternative Name: Total tubulin β; Tubulin beta

Immunogen: Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.

Conjugate: HRP

Modification: Unmodified