Tubulin β Polyclonal Antibody-AP0064

Tubulin β Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0064-50, AP0064-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat, Odontobutis obscurus (not applicable to some tissue)

Applications: WB, IP, IF

All Applications: WB:1:5000~1:20000
IP: 1:100~500
IF: 1:500~1000

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Tubulin β polyclonal antibody pAb detects endogenous levels of Tubulin β protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: MG63 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug)
Lane 6: The Brain tissue lysate of Rat (40ug)
Lane 7: The Brain tissue lysate of Mouse (40ug), Western blot (WB) analysis of Tubulin β pAb at 1:5000 dilution
Lane 1: A549 whole cell lysate (20ug)
Lane 2: PC12 whole cell lysate (20ug)
Lane 3: BV2 whole cell lysate (20ug)
Lane 4: The Kidney tissue lysate of Mouse (20ug)
Lane 5: The Prostate tissue lysate of Rat (20ug), Immunofluorescence analysis of RAW264.7 cells using Tubulin β pAb at dilution of 1:1000 (40x lens)., Immunoprecipitation of MCF-7 cell lysates using Tubulin β pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3). Lane 1 is 30% input. The western blot was probed using Tubulin β mAb.

Alternative Name: Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4

Immunogen: Recombinant full length Human Tubulin β.

Conjugate: Unconjugated

Modification: Unmodified