Product Description

TyrRS Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8516-50, ES8516-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, ELISA

Alternate Names: Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS)

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500

Isotype: IgG

Target: TyrRS

Specificity: TyrRS Polyclonal Antibody detects endogenous levels of TyrRS

Dilution: WB 1:500-2000,IHC 1:50-300 ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: B-cell lymphoma, Bone, Lung, Platelet,

Observed Band (KD): 60kD

Fields: Aminoacyl-tRNA biosynthesis

Function: Catalytic Activity: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Disease: Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM: 608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec
Function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family
Similarity: Contains 1 tRNA-binding domain
Subunit: Homodimer

Subcellular Location: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: YARS

Protein Name: TyrRS

Mouse Gene ID: 107271

Mouse SwissProt Number: Q91WQ3

Rat SwissProt Number: Q4KM49

Research Use Only