Product Description

U2AF1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES9047-50, ES9047-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 60-140

Isotype: IgG

Target: U2AF1

Specificity: U2AF1 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: B-cell lymphoma, Bone marrow, Epithelium, Fetal brain, Lung, Prostate, Sp

Observed Band (KD): 26kD

Fields: Spliceosome, Shigellosis

Function: Domain: The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer
Function: Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Belongs to the splicing factor SR family
Similarity: Contains 1 RRM (RNA recognition motif) domain
Similarity: Contains 2 C3H1-type zinc fingers
Subunit: Identified in the spliceosome C

Subcellular Location: Nucleus Nucleus speckle

Storage and Stability: -20°C/1 year

Gene Name: U2AF1 U2AF35 U2AFBP FP793

Protein Name: Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa subunit) (U2 small nuclear RNA auxiliary factor 1) (U2 snRNP auxiliary factor small subunit)

Mouse SwissProt Number: Q9D883

Research Use Only