Product Description

UBE2B Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES12453-50, ES12453-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human UBE2B AA range: 23-73

Isotype: IgG

Target: UBE2B

Specificity: This antibody detects endogenous levels of UBE2B at Human/Mouse/Rat

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Molecular Weight (Da): 17kD

Fields: Ubiquitin mediated proteolysis

Function: Catalytic Activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth
Pathway: Protein modification, protein ubiquitination
Similarity: Belongs to the ubiquitin-conjugating enzyme family
Subcellular Location: In peripheral neurons, expressed both at the plasma membrane and in nuclei
Subunit: Interacts with RAD18 and UBR2

Subcellular Location: Cell membrane Nucleus In peripheral neurons, expressed both at the plasma membrane and in nuclei

Storage and Stability: -20°C/1 year

Gene Name: UBE2B RAD6B

Protein Name: UBE2B

Mouse Gene ID: 22210

Mouse SwissProt Number: P63147

Rat Gene ID: 81816

Rat SwissProt Number: P63149

Research Use Only