Product Description

Uridine Diphosphate Glucose Pyrophosphorylase (UGP) Activity Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-K857-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes glucose activation before glycogen synthesis, synthesizing 1-phosphate glucose and UTP molecules into UDP-glucose (UDPG). UDPG is the main form of activating enzyme in higher plants and animals, and participates in the anabolic metabolism of glycogen, sucrose and cellulose as a glucosyl donor.
Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm.

Abbreviation: UGP

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.5-70.0 U/L

Tested Sample Types: Animal tissue;Plant tissue

Assay Length: 35 min

Sensitivity: 0.5 U/L

Inter cv: 2.3-3.9%

Intra cv: 1.0-3.2%

Research Use Only