Product Description

VB1 (Vitamin B1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7916-48, ELK7916-96, ELK7916-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Thiamine, Thiamin, Aneurine

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Nutrition Metabolism, Hormone Metabolism

Assay Length: 2h

Sensitivity: 1.7 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Research Use Only