VB1 (Vitamin B1) ELISA Kit - ELK7916

VB1 (Vitamin B1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7916-48, ELK7916-96, ELK7916-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Thiamine; Thiamin; Aneurine

Assay Type: Competitive Inhibition

Sensitivity: 1.7 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Nutrition metabolism; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only