Product Description

VB7 (Vitamin B7) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8278-48, ELK8278-96, ELK8278-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Biotin, Vitamin H, Coenzyme R, Biopeiderm

Reactivity: General

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Nutrition Metabolism, Hormone Metabolism

Assay Length: 2h

Sensitivity: 91.9 pg/mL

Standard: 20000 pg/mL

Detection Range: 312.5-20000 pg/mL

Research Use Only