VMA (Vanillylmandelic Acid) ELISA Kit - ELK8239

VMA (Vanillylmandelic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8239-48, ELK8239-96, ELK8239-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Vanillyl Mandelic Acid

Assay Type: Competitive Inhibition

Sensitivity: 43 ng/mL

Standard: 10000 ng/mL

Detection Range: 156.25-10000 ng/mL

Sample Type: urine and biological agents

Assay Length: 2h

Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Gastroenterology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only