Product Description

VN2R1P Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA36090-50, RA36090-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The CARL-1 monoclonal antibody reacts with human TWEAK, a type II transmembrane TNF superfamily member with high identity to TNF in its extracellular portion. TWEAK transcript is expressed broadly in many adult and fetal tissues, however, the staining of human peripheral blood mononuclear cells with monoclonal antibodies shows a more restricted pattern. While freshly isolated PBMCs do not express detectable levels of TWEAK on their surface, IFN-gamma-stimulated blood monocytes rapidly upregulate TWEAK surface expression. TWEAK is expressed as membrane bound and secreted forms. Interaction of TWEAK with its counter-receptor promotes secretion of IL-8, activation of NF-kappaB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. Initially, DR3 was thought to be a receptor for TWEAK, but further studies have shown that TWEAK could induce apoptosis via receptors distinct from DR3. While TWEAK exhibits overlapping signaling functions to TNF, it is generally less effective in inducing apoptosis, giving rise to its name, TNF-like weak inducer of apoptosis. For detection of human TWEAK by sandwich ELISA, a combination of purified CARL-2 for capture and biotinylated CARL-1 for detection is recommended.

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IF/ICC, ELISA

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight: 83

Protein Molecular Weight (kDa): 83

Storage: -20°C/1 year

Human Gene ID: 344760

Human SwissProt Number: Q8NGV9

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only