Product Description

WAVE1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3702-50, ES3702-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: WASF1, KIAA0269, SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human WAVE1. AA range:91-140

Isotype: IgG

Target: WAVE1

Specificity: WAVE1 Polyclonal Antibody detects endogenous levels of WAVE1 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Highly expressed in brain, Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood,

Observed Band (KD): 70kD

Fields: Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton, Bacterial invasion of epithelial cells, Pathogenic Escherichia coli infection, Shigellosis, Choline metabolism in cancer

Function: Domain: Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain
Function: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton
Similarity: Belongs to the SCAR/WAVE family
Similarity: Contains 1 WH2 domain
Subcellular Location: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas
Subunit: Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2
Tissue Specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small in

Subcellular Location: Cytoplasm, cytoskeleton Cell junction, synapse Cell junction, focal adhesion Dot-like pattern in the cytoplasm Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097) Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503) In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: WASF1

Protein Name: Wiskott-Aldrich syndrome protein family member 1

Mouse Gene ID: 83767

Mouse SwissProt Number: Q8R5H6

Rat Gene ID: 294568

Rat SwissProt Number: Q5BJU7

Research Use Only