Product Description

Xanthine/Hypoxanthine Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F024-48, E-BC-F024-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Xanthine and hypoxanthine are important alkaloid purines that can be used to treat symptoms such as asthma relief and bronchodilation. Hypoxanthine can be oxidized by xanthine oxidase to form xanthine. Xanthine is catalyzed by xanthine oxidase to form uric acid. When xanthine oxidase is deficient, xanthine accumulates in urine and blood, inducing hyperuricemia and gout, and eventually leading to renal failure.
The detection principle of this kit: Xanthine/hypoxanthine generates intermediate products under the catalysis of enzymes. Under the action of other enzymes, the intermediate products react with the probe to form fluorescent substances. By substituting the fluorescence values into the standard curve, the total content of xanthine and hypoxanthine in the sample can be calculated.

Research Areas: Oxidative Stress

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 0.10-50 µmol/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 1 h 50 min

Sensitivity: 0.10 µmol/L

Inter cv: 2.9-8.3%

Intra cv: 0.4-3.2%

Research Use Only