Product Description

XRCC4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3716-50, ES3716-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: XRCC4, DNA repair protein XRCC4, X-ray repair cross-complementing protein 4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human XRCC4. AA range:261-310

Isotype: IgG

Target: XRCC4

Specificity: XRCC4 Polyclonal Antibody detects endogenous levels of XRCC4 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed,

Observed Band (KD): 40kD

Fields: Non-homologous end-joining

Function: Function: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends
PTM: Monoubiquitinated
PTM: Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX
PTM: Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination
Similarity: Belongs to the XRCC4 family
Subunit: Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dep

Subcellular Location: Nucleus Chromosome Localizes to site of double-strand breaks, [Protein XRCC4, C-terminus]: Cytoplasm Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3)

Storage and Stability: -20°C/1 year

Gene Name: XRCC4

Protein Name: DNA repair protein XRCC4

Mouse SwissProt Number: Q924T3

Research Use Only