Zebrafish gABA (Gamma-Aminobutyric Acid) ELISA Kit - ELK0580

Zebrafish gABA (Gamma-Aminobutyric Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0580-48, ELK0580-96, ELK0580-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 4-Aminobutyric Acid

Assay Type: Competitive Inhibition

Sensitivity: 16.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2.5h

Research Areas: Signal transduction; Cardiovascular biology; Neuroscience; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish gABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish gABA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only