ZO-1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A0659-20, A0659-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 195kDa
Observed Molecular Weight: 250kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 7082
SWISS: Q07157
Alternate Names: ZO-1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:2500 - 1:5000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: TJP1
Research Use Only