Product Description

ZO-2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8037-50, ES8037-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: TJP2, X104, ZO2, Tight junction protein ZO-2, Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ZO-2. AA range:1063-1112

Isotype: IgG

Target: ZO-2

Specificity: ZO-2 Polyclonal Antibody detects endogenous levels of ZO-2 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: This protein is found in epithelial cell junctions, Isoform A1 is abundant in the heart and brain, Detected in brain and skeletal muscle, It is present almost exclusively in normal tissues, Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta, Not detected in brain and skeletal muscle, Found in normal as well as in most neoplastic tissues,

Observed Band (KD): 160kD

Fields: Tight junction, Vibrio cholerae infection

Function: Disease: Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM: 607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption
Function: Plays a role in tight junctions and adherens junctions
Similarity: Belongs to the MAGUK family
Similarity: Contains 1 guanylate kinase-like domain
Similarity: Contains 1 SH3 domain
Similarity: Contains 3 PDZ (DHR) domains
Subcellular Location: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures
Subunit: Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus
Tissue Specificity: This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain whereas isoform C1 is expressed at high level in the kidney, pancreas, heart

Subcellular Location: Cell junction, adherens junction Cell membrane, Peripheral membrane protein, Cytoplasmic side Cell junction, tight junction Nucleus Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures

Storage and Stability: -20°C/1 year

Gene Name: TJP2

Protein Name: Tight junction protein ZO-2

Mouse Gene ID: 21873

Mouse SwissProt Number: Q9Z0U1

Research Use Only