Centri-Spin 10 spin columns are used for:

• electroporation nick translation binding applications
• FISH transfection probe labeling gene products
• signal transduction differential display antibody labeling transformation studies
• protein labeling receptor analysis
• solid phase array desalting.

Centri-Spin 10 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)

The Centri-Spin 10 gel will provide excellent recovery of DNA fragments with sizes greater than 10 base pairs or 10-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 5 kD can also be efficiently separated/desalted from unwanted low-molecular-weight impurities.

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Spin 10 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the interstitial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.

CENTRI-SPIN 10 COLUMNS ALSO CAN BE USED FOR THE FOLLOWING APPLICATIONS:

• Purification of primers or probes > 10 bases
• Purification of biotinylation reactions
• Purification of protein conjugates in:

1. iodination
2. cross-linking
3. fluorescence labeling
4. haptenation

• Desalting / purification / buffer exchange of peptides or proteins > 5 kD prior to mass spectroscopy.

These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

ADVANTAGES OF CENTRI-SPIN 10:

• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20–50 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format

BENEFITS OF CENTRI-SPIN 10:

• Reliable and consistent
• Time-saving and convenient
• Enhanced stability
• Extended shelf-life
• No special equipment required
• Extremely versatile