β-Actin (D8) Polyclonal Antibody-AP0731

β-Actin (D8) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0731-50, AP0731-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB:1:5000~1:10000

Background: All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: β-Actin (D8) polyclonal antibody detects endogenous levels of β-actin protein.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:5000 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate
Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:10000 dilution
Lane 4: Hela cell lysate
Lane 5: sp2/0 cell lysate
Lane 6: PC12 cell lysate

Alternative Name: Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β

Immunogen: Recombinant full length Human β-Actin.

Conjugate: Unconjugated

Modification: Unmodified