β-actin Rabbit pAb - EA014

β-actin Rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: EA014-50, EA014-100

Citations, Manuals and MSDS Available upon request.

Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Alternate Name: actin antibody, b-actin antibody, beta-actin antibody

Source: Rabbit

Applications: WB, IHC, IF

Dilution: WB 1:3, 000 IHC 1:200 IF 1:200

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus

Immunogen: Recombinant Protein

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality: Polyclonal

Isotype: IgG

Concentration: 1mg/mL

Observed Band (KD): 43kDa

Human Gene ID: 60

Human SWISS Prot NO: P60709

Subcellular Location: Cytoplasm, Cytoskeleton

Research Use Only