Product Description

μlK1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS80531-50, BS80531-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regμlate the formation of autophagophores, the precursors of autophagosomes. Part of regμlatory feedback loops in autophagy: acts both as a downstream effector and negative regμlator of mammalian target of rapamycin complex 1 (mTORC1 via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regμlator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regμlate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granμle cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regμlate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: μlK1, ATG1, ATG1A, UNC51, Unc51.1, hATG1,μlk1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of humanμlK1(NP_003556.2).

Dilution: WB, 1:500 - 1:1000 IF/ICC, 1:10 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Molecular Weight: 150kDa/140kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O75385

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only