14-3-3γ Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90006-50, BS90006-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P61981(Human) P61982(Mouse) P61983(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, FC
All Applications: WB:1:1,000-1:2,000
FC:1:10-1:50
Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: 14-3-3γ polyclonal antibody detects endogenous levels of 14-3-3γ protein.
Bioworld Molecular Weight: 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: A431
Lane 3: Hela, Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Alternative Name: KCIP-1, YWHAG, 14 3 3 gamma, 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 14-3-3 protein gamma, 1433G, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP-1, KCIP1, N-terminally processed, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide, Ywhag,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified