14-3-3 Theta Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90004-50, BS90004-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P27348(Human) P68254(Mouse) P68255(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
FC:1:50-1:100
Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: 14-3-3 Theta polyclonal antibody detects endogenous levels of 14-3-3 Theta protein.
Bioworld Molecular Weight: 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7, ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: YWHAQ, 14-3-3, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, 1C5, HS1, theta polypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified