Product Description

4E-BP1 (phospho-T37) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4746-50, BS4746-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The translation of proteins from eukaryotic mRNA is initiated by the mμltisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regμlatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regμlated by insμlin 1, PHAS-I, EIF4EBP1, 4EBP1, PHAS I, PHASI

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 37.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-4E-BP1 (T37) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr37.

Molecular Weight: ~ 15 to 20 kDa

Pathway: PI3K AKT signaling, Hypoxia Signaling, Insμlin &Glucose Signaling, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13541

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only