Product Description

4E-BP1 (Phospho-Thr70) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0155-50, AP0155-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and resμlts in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regμlate 4E-BP1 activity. Mμltiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regμlated by insμlin 1, PHAS-I, EIF4EBP1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 70.

Dilution: WB: 1:1000~1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: 4E-BP1(Phospho-Thr70) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr70.

Molecular Weight: ~ 18 kDa

Pathway: PI3K AKT signaling, Hypoxia Signaling, Insμlin &Glucose Signaling, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13541

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only