Product Description

4EBP1 (Phospho-T46) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66300-50, MB66300-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and resμlts in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regμlate 4E-BP1 activity. Mμltiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regμlated by insμlin 1, PHAS-I

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of 4EBP1 (pT46) protein.

Molecular Weight: ~ 15 kDa

Pathway: AMPK Signaling Pathway, Tumor Angiogenesis, Insμlin &Glucose Signaling, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase, MTOR signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13541

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only