53BP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90008-50, BS90008-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q12888(Human) P70399(Mouse) EntrezGene:296099(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: 53BP1 polyclonal antibody detects endogenous levels of 53BP1 protein.
Bioworld Molecular Weight: 214 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining 53BP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining 53BP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: 53BP1, p53-binding protein 1, p53BP1, TP53BP1, 53 BP1, 53BP1, FLJ41424, MGC138366, p202, p53 binding protein 1, p53 BP1, p53-binding protein 1, p53BP1, TP53 BP1, TP53B, Tp53bp1, TRP53 BP1, Tumor protein 53 binding protein 1, Tumor protein p53 binding protein 1, Tumor suppressor p53 binding protein 1, Tumor suppressor p53-binding protein 1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified