Product Description

53BP1 Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA21504-50, RA21504-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage., PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding., PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation., similarity:Contains 2 BRCT domains., subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks., subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3.,

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-p, IF/ICC, ELISA

Alternate Names: TP53BP1, Tumor suppressor p53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight: 213

Protein Molecular Weight (kDa): 213

Storage: -20°C/1 year

Human Gene ID: 7158

Human SwissProt Number: Q12888

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only