Product Description

6-Phosphogluconate Dehydrogenase (6-PGDH) Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K1150-M-48, E-BC-K1150-M-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Category: Cell Metabolism Assays

Alternate Names: 6-PGDH

Storage and Stability: This product can be stored at -20°C for 12 months with shading light.

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Enzyme activity

Detection Range: 1.26-101.85 U/L

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Tested Sample Types: Serum;Plasma;Animal and plant tissue;Cell

Assay Length: 50 min

Sensitivity: 1.26 U/L

Test Principle: 6-phosphogluconate dehydrogenase (6-PGDH) is the rate-limiting enzyme of the pentose phosphate pathway, which is responsible for the production of NADPH, energy balance, growth rate, and cell viability. 6-PGDH is also associated with a variety of tumor signaling pathways, including epidermal growth factor signaling pathway and nuclear factor E2 related factor signaling pathway.
The principle of this kit is that the product generated by the 6-PGDH catalytic substrate reacts with the chromogenic agent to generate the chromogenic substance, which has the maximum absorption at 450 nm, and is calculated by measuring the OD value at 450 nm 6-PGDH enzyme activity.

Inter CV: 5.9-9.4%

Intra CV: 2.9-4.6%

Research Use Only