Product Description

AAK1 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP00069-20, BT-AP00069-50, BT-AP00069-100

Citations, Manuals and MSDS Available upon request.

Background: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 encodes a member (AP2 associated kinase 1) of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Research Areas: Signal Transduction

Target Protein: AAK1

Applications: WB, IHC-p, IF, ELISA

Reactivity: Human, Mouse, Rat, Monkey

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: AAK1 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 93578

UniProt Accession: Human: Q2M2I8; Mouse: Q3UHJ0; Rat: P0C1X8

Synonyms: AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1

GeneID: Human: 22848; Mouse: 269774

Research Use Only