AAK1 rabbit pAb - ES1557

AAK1 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES1557-50, ES1557-100

Citations, Manuals and MSDS Available upon request.

Background: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],

Alternate Name: AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Reactivity: Human; Mouse; Rat; Monkey

Immunogen: The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 94kD

Human Gene ID: 22848

Human SWISS Prot NO: Q2M2I8

Subcellular Location: Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse, presynapse. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.

Research Use Only