ABCA1 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60011-50, BS60011-100
Sizes: 50µl, 100µl
Swiss-Prot: O95477
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: ABC1 (for ATP-binding cassette transporter 1) is a member of the family of ATP-binding cassette proteins which transport various molecules across biological membranes. ABC1 contains two characteristic ATP-binding domains and 12 transmembrane domains which form a channel-like structure for transport. Mutations in the ABC1 gene are implicated in Tangier disease, characterized by low serum high density lipoprotein. ABC1 is widely expressed in human tissues, with high levels of expression in liver, lung, adrenal glands, placenta and fetal tissue. ABC1 expression is induced during monocyte differentiation and upregulated in the presence of acetylated low-density lipoprotein. ABC1 may have a dual regulatory function in macrophage lipid metabolism and inflammation.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ABCA1 polyclonal antibody detects endogenous levels of ABCA1 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ABCA1 polyclonal antibody at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: AML-12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: HepG2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ABCA1 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 254 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: ATP-binding cassette sub-family A member 1;
ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; ABCA1; ABC1, CERP
Immunogen: A synthetic peptide corresponding to residues in Human ABCA1.
Conjugate: Unconjugated
Modification: Unmodified