ABCA7 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS77650-50, BS77650-100
Swiss-Prot: Q8IZY2
Host: Rabbit
ABCA7 Polyclonal Antibody-BS77650
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Unmodification
Bioworld Molecular Weight: 260KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of Rat brain, using ABCA7 Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of U-251 MG cells using ABCA7 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ABCA7; ABCA-SSN; ABCX; AD9
Immunogen: Recombinant fusion protein of human ABCA7(NP_061985.2).
Modification: Unmodified
Conjugate: Unconjugated