ABCC5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS7475-50, BS7475-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O15440
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB,1:1000 - 1:2000
Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing results in multiple transcript variants.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 185kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot analysis of extracts of various cell lines, using ABCC5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Alternative Name: ABCC5; ABC33; EST277145; MOAT-C; MOATC; MRP5; SMRP; pABC11
Immunogen: Recombinant fusion protein of human ABCC5(NP_005679.2).
Conjugate: Unconjugated
Modification: Unmodified